Influence of synthetic peptides, corresponding to fragments of the human α‐2 interferon molecule, on the proliferation of lymphoblastoid cells in vitro. Growth inhibition and receptor binding

[摘要]

Influence of nine synthetic peptides from the C-terminal part of human α-2 interferon (EN) molecule on growth of a human T-lymphoblastoid cell line MT-4 was investigated. It was shown that some peptides inhibited the proliferation of MT-4 cells. It was also found that MT-4 cells expressed specific receptors on the outer surface of the plasma membrane. All studied peptides competed for binding to a common binding site. Antiproliferative activity of the peptides correlated with their length and affinity to receptors on the outer membrane of MT-4 cells.

[学科分类] 生物化学/生物物理

[关键词] Synthetic peptide;Interferon α;Tumor cell;Growth inhibition;Receptor;IFN;interferon;[3H]TdR;[3H]thymidine;K d;equilibrium dissociation constant;IC50;concentration of competing ligand causing half-maximum displacement;K i;inhibition constant