[摘要] Stimulation of cells by epidermal growth factor induces a rapid polymerisation of actin in the cortical skeleton. Activation of the EGF-receptor leads to autophosphorylation and to phosphorylation of specific intracellular substrates. Here we show that actin is phosphorylated in vitro and in vivo upon EGF stimulation. Two-dimensional phospho-amino acid analysis shows that phosphorylation occurs on serine, not on tyrosine residues.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] EGF;EGF-receptor;Actin;Phosphorylation;ATP;adenosine triphosphate;DMEM;Dulbecco;modified Eagle's medium;EDTA;ethylene diamide tetra acetic acid;EGF;epidermal growth factor;FCS;fetal calf serum;NGF;nerve growth factor;PDGF;platelet-derived growth factor;PKA;protein kinase A;PKC;protein kinase C;PMSF;phenylmethylsulfonyl fluoride;SDS;sodium dodecyl sulphate;SDS-PAGE;sodium dodecyl sulphate-polyacrylamide gel electroforesis;TCA;trichloroacetic acid;TLC;thin layer chromatography [时效性]
