Ribosomal protein L22 from Thermus thermophilus: sequencing overexpression and crystallisation

[摘要]

The gene for the ribosomal protein L22 from Thermus thermophilus has been sequenced and overexpressed in Escherichia coli. A multiple sequence alignment was carried out for all proteins of the L22 family reported so far. The recombinant protein was purified and crystallized. The crystals belong to the space group P2₁2₁2₁ with cell parameters of .

[学科分类] 生物化学/生物物理

[关键词] Ribosomal protein;Overexpression;Purification;Crystallisation;Thermus thermophilus