NMR and circular dichroism studies of synthetic peptides derived from the third intracellular loop of the β‐adrenoceptor

[摘要]

The C-terminal part of the third intracellular loop of the β-adrenoceptor is capable of stimulating adenylate cyclase in the presence of phospholipid vesicles via the stimulatory guanine nucleotide binding protein (G_s) [Palm et al. (1989) FEBS Lett. 254, 89–93]. We have investigated the structure of synthetic peptides corresponding to residues 284–295 of the turkey erythrocyte adrenoceptor in micelles, trifluoroethanol and aqueous solution, by using 2D ¹H NMR and CD. In the presence of phospholipid micelles the peptides display a C-terminal α-helical region, whereas the N-terminal part was found to be highly flexible.

[学科分类] 生物化学/生物物理

[关键词] β-Adrenoceptor;Circular dichroism;Conformational change;1H NMR;Peptide-phospholipid interaction;2D;3D;two-;three-dimensional;CD;circular dichroism;COSY;correlated spectroscopy;DIC;diisopropylcarbodiimide;G-protein;guanine nucleotide binding regulatory protein;Gs protein;stimulatory G protein;Go protein;a G protein from brain;HOBT;1-hydroxybenzotriazole;HPLC;high performance liquid chromatography;MALDI-TOF;matrix assisted laser desorption/ionisation-time of flight;NMR;nuclear magnetic resonance;NOESY;nuclear Overhauser enhancement spectroscopy;lyso-PC;lyso-phosphatidylcholine;ROESY;rotating frame Overhauser enhancement spectroscopy;TFA;trifluoroacetic acid;TFE;trifluoroethanol;TFE-d3;perdeuterated trifluoroethanol;TOCSY;total correlation spectroscopy;TSP;3-trimethylsilyltetradeuteropropionate