[摘要] We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected. The phage genome encodes a fusion of single-chain antibody to the C-terminal domain of gIIIp, rendering the phage non-infective. The antigen fused to the N-terminal domains of gIIIp is encoded in the same phage genome. Antigen and antibody fusion interact with each other in the periplasm of the phage-producing cell, restoring infectivity. This system has a very low background and will allow simultaneous randomisation of antibody and antigen.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Selectively-infective phage;Phage display;Antibody engineering;Filamentous phage;Single-chain Fv fragment;aa;amino acid;amp;ampicillin;cam;chloramphenicol;cfu;colony forming units;ELISA;enzyme linked immunosorbent assay;HAG;hemagglutinin;hag;peptide from hemagglutinin bound by antibody 17/9;kan;kanamycin;OD550;optical density at 550 nm;PBS;phosphate-buffered saline;PCR;polymerase chain reaction;pfu;plaque forming units;scFv;single-chain Fv fragment;SIP;selectively-infective phage;tet;tetracycline;VH;variable domain of the heavy chain;VL;variable domain of the light chain;wt;wild type [时效性]
