DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells

[摘要]

Treatment of human respiratory tract epithelial cells with H₂O₂ led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH^.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

[学科分类] 生物化学/生物物理

[关键词] Human respiratory tract epithelial cell;DNA damage;DNA base modification;Strand breakage;H2O2;GC-MS;5-OH-uracil;5-hydroxyuracil;5-OHMe-uracil;5-(hydroxymethyl)uracil;FAPy-adenine;4;6-diamino-5-formamidopyrimidine;8-OH-adenine;8-hydroxyadenine;FAPy-guanine;2;6-diamino-4hydroxy-5-formamidopyrimidine;8-OH-guanine;8-hydroxyguanine;8-OHdG;8-hydroxy-2′-deoxyguanosine