[摘要] The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Immunoliposome;Monoclonal antibody;ICAM-1;Epithelium;Endothelium;BSA;bovine serum albumine;CHOL;cholesterol;DMF;dimethylformamide;EDTA;ethylene diaminetetraacetic acid;EPG;egg-phosphatidylglycerol;FCS;foetal calf serum;GamFITC;fluorescein isothiocyanate-labeled goat anti-mouse mAb;HBS;HEPES-buffered saline;HUVEC;human umbilical vein endothelial cells;ICAM-1;intercellular adhesion molecule-1;IFN-γ;interferon-γ;MPB-PE;N-[4-(p-maleimidophenyl)butyryl] phosphatidylethanolamine;MFI;mean fluorescence intensity;mAb;monoclonal antibody;PBS;phosphate-buffered saline;PHEPC;partially hydrogenated egg l-α-phosphatidylcholine;PL;phospholipid;SATA;N-succinimidyl S-acetylthioacetate;TNF-α;tumor necrosis factor-α;VCAM-1;vascular cell adhesion molecule-1 [时效性]
