Novel avidin and streptavidin binding sequences found in synthetic peptide libraries
[摘要] Synthetic resin-bound peptide libraries made of protein l-amino acids have been synthesized. During screening of the libraries, peptides that bind to avidin have been identified containing a novel motif with two histidines separated by one residue. A sub-library was synthesized and screened, and new critical residues appeared surrounding the two histidines. Additionally peptide libraries made of d-amino acids have been screened with avidin and streptavidin and novel motifs have been found.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Synthetic peptide library;Sub-library;Avidin;Streptavidin;Molecular recognition;BCIP;5-bromo-4-chloro-3-indolyl phosphate;BSA;bovine serum albumin;DAB;diaminobenzidine;DIEA;diisopropylethylamine;DMF;N;N-dimethylformamide;EDT;ethandithiol;ELISA;enzyme-linked immunosorbent assay;Fmoc;9-fluorenylmethoxycarbonyl;HPLC;high performance liquid chromatography;HOBt;1-hydroxybenzotriazole;NBT;nitro blue tetrazolium;OPD;o-phenylenediamine;PBS;phosphate-buffered saline;Pfp;pentafluorophenyl;SPDP;3-(2-Pyridyldithio)propionic acid N-hydroxy-succinimide ester;TBTU;2-(1H-benzotriazole-1-yl)-1;1;3;3-tetramethyluronium tetrafluoroborate);TFA;trifluoracetic acid [时效性]
