The targeting information of the mitochondrial outer membrane isoform of cytochrome b5 is contained within the carboxyl‐terminal region

[摘要]

Two isoforms of mammalian cytochrome b ₅, which have homologous cytosolic amino-terminal catalytic domains, are located one on endoplasmic reticulum (ER b ₅) the other on mitochondrial outer membranes (OM b ₅). A cDNA coding for the previously unknown carboxyl-terminal domain of OM b ₅ was cloned and a chimera between the catalytic domain of ER b ₅ and the carboxyl-terminal region of OM b ₅ was expressed in cultured mammlian cells. The chimera localized to mitochondria, indicating that the carboxyl-terminal 43 amino acids of OM b5 contain sufficient information to target the catalytic domain of ER b ₅ to the mitochondrial outer membrane.

[学科分类] 生物化学/生物物理

[关键词] Endoplasmic Reticulum;Cytochrome b5;Membrane biogenesis;Membrane protein;Mitochondrial outer membrane;Protein targeting;cyt;cytochrome;ER;endoplasmic reticulum;ER b 5;endoplasmic reticulum isoform of cytochrome b 5;NER-COM;chimera between N-terminal domain of ER b 5 and C-terminal domain of OM b 5;OM b 5;outer mitochondrial membrane isoform of cytochrome b 5;PDI;protein disulphide isomerase