[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Disulfide bond formation;Protein stability;Thermal denaturation;barstarox;barstar with intramolecular disulfide bridge;barstar(E80A);barstar mutant with amino acid substitution glutamate80 → alanine80;barstar(C40A/C82A);barstar mutant with both cysteines substituted by alanines;CD;circular dichroism;DTNB;5;5'dithiobis-(2-nitrobenzoic acid);DTT;dithiothreitol;GdnHCl;guanidine hydrochloride;GSH and GSSG;thiol and disulfide form of glutathione;PAGE;polyacrylamide gel electrophoresis;SDS;sodium dodecyl sulphate;Tris-HCl;Tri(hydoxymethyl)aminomethane-hydrochloride;UV;ultraviolet [时效性]
