Induction of vascular endothelial growth factor expression in synovial fibroblasts by prostaglandin E and interleukin‐1: a potential mechanism for inflammatory angiogenesis

[摘要]

Inflammatory mediators such as prostaglandin E₂ (PGE₂) and interleukin-1 (IL-1) induce angiogenesis by yet undefined mechanisms. We demonstrate that PGE₂ and IL-1 induces the expression of vascular endothelial growth factor (VEGF), a selective angiogenic factor by rheumatoid synovial fibroblast cells. Transcripts for the EP₁ and EP₂, subtypes of PGE receptors are expressed in synovial fibroblasts. Activators of protein kinase A pathway stimulated the expression of VEGF whereas down-regulation of protein kinase C did not influence the PGE effect, suggesting that signalling from the EP₂ receptor via the protein kinase A pathway is important. The induction of VEGF expression by PGE₂ and interleukin-1α a may be an important mechanism in inflammatory angiogenesis.

[学科分类] 生物化学/生物物理

[关键词] Angiogenesis;Interleukin-1α;Prostaglandin E2;Rheumatoid arthritis;Vascular endothelial growth factor;IL-1α;interleukin-1;PGE1;prostaglandin E1;PGE2;prostaglandin E2;RA;rheumatoid arthritis;RT-PCR;reverse transcriptase-polymerase chain reaction;VEGF;vascular endothelial growth factor