7‐O‐hemisuccinyl‐deacetyl forskolin—sepharose: a novel affinity support for purification of adenylate cyclase

[摘要]

[学科分类] 生物化学/生物物理

[关键词] Adenylate cyclase;Forskolin;Affinity chromatography;Reconstitution;Guanine nucleotides;Gpp(NH)p;guanosine 5′(β-imino)triphosphate;MOPS;3-(N-morpholino)propanesulfonic acid;EGTA;ethylene glycol bis(β-aminoethylether)- N;N′-tetraacetic acid