Identification of the active site cysteine and of the disulfide bonds in the N‐terminal part of the molecule of bovine spleen cathepsin B
[摘要]
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] RAE-;reduced and aminoethylated-;dansyl-;1-dimethylaminonaphthalene-5-sulfonyl-;DABTH-;4-N;N-dimethylaminoazobenzene-4′-thiohydantoin derivative;PTH-;phenylthiohydantoin derivative;HPLC;high-performance liquid chromatography;EDTA;ethylenediamine tetraacetic acid;DTNB;5;5′-dithiobis (2-nitrobenzoic acid);DTT;dithiothreitol;SDS—PAGE;sodium dodecyl sulfate—polyacrylamide gel electrophoresis [时效性]
