Undegraded polysomes could be extracted from eggs of Bombyx mori by cutting egg shells with a blade in a buffer containing high salt. The polysome content as measured by this method increased steeply during post-diapause development, which was commenced by long term chilling followed by hot HCl treatment of diapausing eggs. At the 24th hr of the post-diapause development the polysome content became 2.6-times the level at 0 h. The alteration of the polysome content paralleled that of the incorporation of [14C]leucine into acid-insoluble fractions investigated by modified Takami's embryonic culture system.
