Base‐pair formation between 18 S ribosomal RNA and globin mRNA during initiation of protein synthesis in vitro

[摘要]

A stable complex between 18 S rRNA and globin mRNA has been isolated from 40 S initiation complexes in the reconstituted reticulocyte cell free system. This complex is only formed under the conditions which also lead to an initiation complex active in protein synthesis. The mRNA—18 S rRNA interactions has properties compatible with base-pairing. This observation is discussed in the context with other, in part controversial, observations relating to base pairing as a step in initiation of eukaryotic protein synthesis.

[学科分类] 生物化学/生物物理

[关键词] Base pairing;Initiation factor;mRNA—18 S rRNA interaction;Protein synthesis;eukaryotic;Reconstitution;in vitro;Ribosome;eIF;eukaryotic initiation factor;LDS;lithium dodecylsulfate;Hepes;4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid