(poly)Phosphoinositide phosphorylation is a marker for plasma membrane in Friend erythroleukaemic cells

[摘要]

Upon subcellular fractionation of (murine) Friend erythroleukaemic cells (FELCs), purified plasma membranes were identified by their high enrichment in specific marker enzymes and typical plasma membrane lipids. When FELCs were incubated for short periods with ³²P_i before cell fractionation, the lipid-bound radioactivity was almost exclusively present in phosphatidylinositol-4-phosphate (DPI) and phosphatidylinositol-4,5-bisphosphate (TPI), and its distribution closely matched that of the plasma membrane markers. In addition, purified plasma membranes actively incorporated ³²P from [γ-³²P]ATP into polyphosphoinositides, and the specific activities of the involved kinases were again mostly enriched in the plasma membrane fraction.

[学科分类] 生物化学/生物物理

[关键词] Friend erythroleukaemic cells;Cell fractionation;Marker enzymes;Plasma membrane;labeling;(poly) Phosphoinositide phosphorylation;FELC;Friend erythroleukaemic cells;DPI;phosphatidylinositol-4-phosphate;TPI;phosphatidylinositol-4;5-bisphosphate;PA;phosphatidate;PI;phosphatidylinositol