Involvement of calcium, calmodulin and phospholipase A in the alteration of membrane dynamics and superoxide production of human neutrophils stimulated by phorbol myristate acetate

[摘要]

We have reported an increased fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in the phorbol myristate acetate-stimulated plasma membrane of human neutrophils [FEBS Lett. (1982) 144, 199–203]. We now present evidence that both the increased fluorescence polarization and the production of super-oxide radicals by human neutrophils require calcium, calmodulin and phospholipase activity.

[学科分类] 生物化学/生物物理

[关键词] Membrane dynamics;Order parameter;Neutrophil;Calcium;Calmodulin;Phospholipase A;DPH;1;6-diphenyl-1;3;5;-hexatriene;EGTA;ethylene glycol-bis (β-amino ethylether-N;N'-tetraacetic acid;PMA;phorbol-12-myristate-13-acetate;PMN;polymorphonuclear leucocytes