Muscle glycolipids in inherited muscular dystrophy of chickens

[摘要]

Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chickens. Based on the thin-layer chromatographic mobility, treatment with neuraminidases from Vibrio cholerae and Arthrobacter ureafaciens, and reactivity of the asialo-derivative towards anti-ganglio-N-triaosylceramide antibody, the dystrophic-specific ganglioside was tentatively identified as G_M2. Data obtained from young and old dystrophic chickens suggested a direct relationship of this ganglioside to muscular dystrophy.

[学科分类] 生物化学/生物物理

[关键词] Ganglioside;Neutral glycolipid;Chicken muscular dystrophy;GM2;Degenerating muscle;Dystrophic-specific ganglioside;CMH;galactosyl or glucosylceramide;CDH;lactosylceramide;CTH;globotriaosylceramide;LT;lactotriaosylceramide GlcNAc(β1–3)Gal(β1–4)-Glc-Cer;As GM2;ganglio-N-triaosylceramide;PG;lactoneotetraosylceramide Gal(β1–4)GlcNAc(β1–3)Gal-(β1–4)Glc-Cer;As GM1;ganglio-N-tetraosylceramide;Forss;Forssman glycolipid;SPG;sialosyllactoneotetraosylceramide NeuAc(α2–3)Gal(β1–4)GlcNAc(β1–3)-Gal(β1–4)Glc-Cer;SNH;sialosyllacto-N-nor-hexaosylceramide NeuAc(α2–3)[Gal(β1–4)GlcNAc(β1–3)]2Gal-(β1–4)Glc-Cer