The hydroperoxide‐induced release of mitochondrial calcium occurs via a distinct pathway and leaves mitochondria intact

[摘要]

The hydroperoxide-induced net release of Ca²⁺ from rat liver mitochondria is stimulated by the Ca²⁺ uptake inhibitor ruthenium red. At moderate Ca²⁺ loads the release takes place with preservation of a high mitochondrial membrane potential. During and after Ca²⁺ release mitochondria remain intact. The hydroperoxide-induced release of Ca²⁺ might therefore be a physiological relevance.

[学科分类] 生物化学/生物物理

[关键词] Mitochondria;Calcium release;Membrane potential;Intactness;Ruthenium red;ΔΨ;mitochondrial transmembrane electrical potential;negative inside;ΔE;deflection of the tetraphenyl-phosphonium electrode from the baseline;arsenazo III;2;2'-(1;8-dihydroxy-3;6-disulphonaphthalene-2;7-bis azo)bis(benzenarsonic acid);CCCP;carbonyl-cyanide m-chlorophenylhydrazone;EGTA;ethyleneglycol bis (β-aminoethylether)-N;N;N';N'-tetraacetic acid;Hepes;4-(2-hydroxyethyl)-1-piperazinemethene-sulphonic acid;ruthenium red;Ru2(OH)2Cl4 · 7 NH3 · 3 H2O;TPP+;tetraphenylphosphonium