Non‐vectorial phosphorylation by the bacterial PEP‐dependent phosphotransferase system is an artifact of spheroplast and membrane vesicle preparation procedures

[摘要]

These data demonstrate that the phenomenon of non-vectorial phosphorylation [Saier, M.H. jr and Schmidt, M.R. (1981) J. Bacteriol. 145, 391–397] is an experimental artifact which arises during the conversion of whole cells to spheroplasts or membrane vesicles. It can be explained by a certain population of particles which are not properly sealed. There is no need to envoke two orientations of enzyme II in the membrane, one of which would have no physiological significance.

[学科分类] 生物化学/生物物理

[关键词] Enzyme II orientation;Vectorial phosphorylation;Hexose transport;Sealed versus leaky particle;Phosphotransferase;PEP-dependent;Experimental artifact