Changes in phospholipids during the cell cycle of myxomycete Physarum polycephalum

[摘要]

The rate of ³²P_i incorporation into the main membrane phospholipid fractions, i.e. phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), as well as their contents in the cells during synchronous growth of the myxomycete, Physarum polycephalum, have been studied. It has been found that both the phospholipid levels and the rates of ³²P_i incorporation increase during the S phase till the early G₂ phase, remain nearly constant during the G₂ phase and fall to the initial level at the end of the G₂ phase and in mitosis. It has been revealed that the rate of ³²P_i incorporation into PC is very low compared to PE and PI, in spite of the fact that the PC level is the highest. The possible reasons of this phenomenon are discussed.

[学科分类] 生物化学/生物物理

[关键词] Cell cycle;Phospholipid;Physarum polycephalum;PC;phosphatidylcholine;PE;phosphatidylethanolamine;PI;phosphatidylinositol;32Pi;[32P]orthophosphoric acid