A novel gene delivery system using EGF receptor‐mediated endocytosis

[摘要]

A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF receptor-overproducing squamous cell carcinomas.

[学科分类] 生物化学/生物物理

[关键词] EGF receptor;Endocytosis;Monoclonal antibody;Gene therapy;EGF;epidermal growth factor;MoAb;monoclonal antibody;CAT;chloramphenicol acetyltransferase;SPDP;N-succinimidyl 3-(2-pyridyldithio) propionate;TNF;tumor necrosis factor;3-AcCM;3-acethyl chloramphenicol;CM;chloramphenicol;EBSS;Earle's balanced salt solution