Yeast serine isoacceptor tRNAs: Variations of their content as a function of growth conditions and primary structure of the minor tRNASerGCU

[摘要]

The primary structure of Saccharomyces cerevisiae tRNA^SerGCU is presented (EMBL database accession No. X74268 S. cerevisiae tRNA-Ser). In addition, quantitation of the relative amounts of serine isoaccepting tRNAs in yeast grown on different media showed that the minor tRNA^SerGCU decreased while the major tRNA^SerAGA increased as the growth rate and the cellular protein content increased. The minor species, tRNA^SerCGA and tRNA^SerUGA, were not separated by our gel system, however, taken together they appeared to vary in the same way as TRNA^SerGCU. These data suggest a growth rate dependence of yeast tRNAs similar to that previously described for E. coli tRNAs.

[学科分类] 生物化学/生物物理

[关键词] Saccharomyces cerevisiae;tRNAserGCU primary structure;Serine isoaccepting tRNA abundance