SNAP‐25 is present on chromaffin granules and acts as a SNAP receptor

[摘要]

SNAP-25 is located on the plasma membrane and essential for exocytosis of neurotransmitters. It was suggested that SNAP-25 and syntaxin 1 via the interaction with VAMP-2 located on synaptic vesicles mediate the docking of the vesicles with the plasma membrane. In the present study, by means of biochemical and morphological analyses, we showed that SNAP-25 is present on chromaffin granules as well as on the plasma membrane. Reconstitution and immunoprecipitation analyses revealed that SNAP-25 on chromaffin granules has essentially the same properties as does SNAP-25 on the plasma membrane.

[学科分类] 生物化学/生物物理

[关键词] Chromaffin granule;N-Ethylmaleimide-sensitive factor;Soluble NSF attachment protein;NSF;N-ethylmaleimide-sensitive factor;SNAP;soluble NSF attachment protein;SNARE;SNAP receptor;MAb;monoclonal antibody;PBS;phosphate-buffered saline;BSA;bovine serum albumin;PAGE;polyacrylamide gel electrophoresis