Acidic phospholipids inhibit the phospholipase D activity of rat brain neuronal nuclei

[摘要]

An oleate dependent form of phospholipase D is present in rat brain neuronal nuclei and both the hydrolytic and transphosphatidylation activities measured. Several acidic phospholipids were found to inhibit this activity in a dose dependent manner. The IC₅₀ values varied from 3.5 μM for PIP₂ to 200 μM for phosphatidic acid. The hydrolysis of PIP₂ by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.

[学科分类] 生物化学/生物物理

[关键词] Phospholipase D;Neuronal nuclei;Phosphatidic acid;Phosphatidylinositol-4;5-diphosphate;Acidic phospholipids;PLD;phospholipase D;PtdCho;phosphatidylcholine;PtdSer;phosphatidylserine;Ptdglycerol;phosphatidylglycerol;PtdE-tOH;phosphatidylethanol;PtdA;phosphatidic acid;PIP2;phosphatidylinositol-4;5-diphosphate;PtdIns;phosphatidylinositol;PLC;phospholipase C;ARF;ADP ribosylation factor