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Isolation and characterization of a cDNA encoding a sulfate transporter from Arabidopsis thaliana
[摘要]

A cDNA encoding a sulfate transporter was isolated from Arabidopsis thaliana. The isolated clone contained an open reading frame encoding a polypeptide of 658 amino acids, exhibiting the highest similarity (62%) with the sequence of the low-affinity sulfate transporter of a tropical legume Stylosanthes hamata. Northern blot analysis indicated the constitutive accumulation of a 2.6 kb length transcript in leaves and roots of seedlings. We also propose that A. thaliana contains three sulfate transporter genes which are expressed as 3.0, 2.7 and 2.6 kb length transcripts, respectively, in an organ-specific manner.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Arabadopsis thaliana;cDNA cloning;Expressed sequence tag;Sulfate transporter;AST;Arabidopsis thaliana sulfate transporter;EST;expressed sequence tag;MSD;membrane-spanning domain [时效性] 
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