Bandeiraea (Griffonia) simplicifolia lectin‐I, isolectin A4, reacting with Tn (GalNAαl → Ser/Thr or galabiose (Galα1 → 4Gal) containing ligands

[摘要]

Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A₄(GS I-A₄) reacting with the Tn(GalNAcα1 → Ser/Thr) sequence or human blood group P ^k active dissacharide (E, Galα1 → 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P₁ or Tn active glycoproteins were tested by QPA, GS IA₄ reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P₁ active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A₄ interaction was inhibited by both Tn containing glycopeptides and Galα1 → 4Gal indicating that GS I-A₄ not only reacts with human blood group A(GalNAcα1 → 3Gal) and B(Galα1 → 3Gal) active dissacharides, but also recognizes the Tn sequence and the E(Galα1 → 4Gal) ligand. From these results, the carbohydrate specificity of GS I-A₄ can be defined as A, Tn ≥ B and E.

[学科分类] 生物化学/生物物理

[关键词] Bandeiraea (Griffonia) simplicifolia lectin-I A4;Tn-active glycoprotein;Sheep hydatid cyst glycoprotein;Binding property;Gal;d-galactopyranose;Glc;d-glucopyranose;lFuc or Fuc;l-fucopyranose;GalNAc;2-acetamido-2-deoxy-d-galactopyranose;GlcNAc;2-acetamido-2-deoxy-d-glucopyranose;GS I-A4 (BS I-A4);Griffonia (Bandeiraea) simplicifolia lectin-I A4;GP;glycoprotein;THGP;Tamm-Horsfall glycoprotein;MSM;one of human blood group A active glycoproteins purified from human ovarian cyst fluid [26];OSM;ovine submandibular glycoprotein-major;ASG-A;armadillo submandibular glycoprotein fraction A [17] which is one of the sialic acid containing glycoproteins in armadillo submandibular glands;native ASG-Tn [18];a naturally occurring Tn glycoprotein isolated from the extract of armadillo submandibular glands in 0.01 M PBS pH 6.8 after removal of ASG-A;PSM;porcine salivary GPmajor;BSM;bovine submandibular GP-major;QPA;quantitative precipitin assay;QPIA;quantitative precipitin-inhibition assay. Lectin determinants that are used to classify applied lectins [14;30;31] are expressed in bold: A(GalNAcα1 → 3Gal);b(Galα1 → 3Gal);Tn(GalNAcα1 → Ser/Thr);E(galabiose Galα1 → 4Gal) [30;31];the human blood group Pk(Galα1 → 4Galβ1 → 4Glc-Cer) active dissacharide or part of P1 determinant structure (Galα1 → 4Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Gcl-Cer) at the terminal non-reducing end