The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase

[摘要]

Macrophages contain arginase and an inducible NO synthase, demonstrated by using l-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite with arginine, or by the irreversible covalent modification of arginase, or by the removal of Mn²⁺, a cofactor of arginase.

[学科分类] 生物化学/生物物理

[关键词] Arginase;NO synthase;Nitrite;Inhibition;Fluorescence;Macrophage;FBS;foetal bovine serum;DMEM;Dulbecco's minimal essential medium;IFNγ;interferon-γ;IL;interleukin;NOS;NO synthase;TLC;thin-layer chromatography;TNFα;tumor necrosis factor-α;amino acids are abbreviated by their usual three-letter symbols