Differential display PCR reveals increased expression of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase by lithium

[摘要]

Differential display PCR was used to study the effects of lithium on gene expression. Four candidate genes were isolated and verified by Northern hybridization after 1 week treatment of C6 glioma cells with therapeutically relevant concentrations of LiCl (1 mM). Sequencing analysis revealed three previously unidentified cDNA fragments in addition to a sequence with 99% homology with the cDNA for 2′,3′-cyclic nucleotide 3′-phosphodiesterase type II (CNPaseII). Since CNPaseII is important in myelinogenesis and possibly neuronal growth and repair, the present findings suggest that lithium treatment may regulate these processes.

[学科分类] 生物化学/生物物理

[关键词] Differential display PCR;Lithium;2′;3′-Cyclic nucleotide 3′-phosphodiesterase;Bipolar disorder;Gene expression;DD-PCR;differential display polymerase chain reaction;CNPase;2′;3′-cyclic nucleotide 3′-phosphodiesterase;LiRG;lithium-regulated gene;MBP;myelin basic protein;G-protein;GTP binding protein;PKC;protein kinase C;CNS;central nervous system;BD;bipolar affective disorder