Maltokinase (ATP: maltose 1‐phosphotransferase) from Actinoplanes sp.: demonstration of enzyme activity and characterization of the reaction product

[摘要]

Cell free extract of the acarbose producer Actinoplanes sp. catalyzes ATP-dependent phosphorylation of maltose. This was shown by two different assays. The product was purified and its structure determined to be α-maltose-1-phosphate by chemical analysis and NMR spectroscopy.

[学科分类] 生物化学/生物物理

[关键词] Maltose;Kinase;Maltose-1-phosphate;NMR;Actinoplanes