Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein‐3

[摘要]

¹H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (∼0.2–2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

[学科分类] 生物化学/生物物理

[关键词] Monocyte chemotactic protein;NMR;Solution structure;Monomer-dimer;IL-8;interleukin-8;MCP;monocyte chemoattractant protein;MGSA;melanoma growth stimulatory activity;MIP-1β;macrophage inhibitory protein-1β;NAP-2;neutrophil activating peptide-2;PF-4;platelet factor-4;DQF-COSY;double quantum filtered correlation spectroscopy;NOE;nuclear Overhauser effect;NOESY;NOE spectroscopy;RANTES;regulated on activation normal T cell expressed;HPLC;high-performance liquid chromatography;RMS;root-mean-square