On the mechanism by which 6‐ketocholestanol protects mitochondria against uncoupling‐induced Ca2+ efflux

[摘要]

This work shows that 6-ketocholestanol (kCh) inhibits the effect of carbonyl cyanide-m-chlorophenyl hydrazone (CCP) on mitochondrial Ca²⁺ efflux. Such an effect proved to be caused by diminution of membrane fluidity, therefore, it is affected by the incubation temperature. Furthermore, kCh reversed CCP-induced Ca²⁺ efflux depending on the accumulation of phosphate. It is also shown that kCh enhances the effect of carboxyatractyloside on membrane permeability transition.

[学科分类] 生物化学/生物物理

[关键词] Mitochondrial Ca2+;Calcium;Mitochondrion (kidney);Permeability transition;6-Ketocholestanol;Mitochondrial membrane;kCh;6-ketocholestanol;CCP;carbonyl cyanide-m-chlorophenyl hydrazone;CAT;carboxyatractyloside;Pi;inorganic phosphate;MD;menadione;DPH;1;6-diphenyl-1;3;5-hexatriene;ANS;8-aniline-naphthalene sulfonic acid