Direct excision of 50 kb pair DNA fragments from megabase‐sized fragments produced during apoptotic cleavage of genomic DNA

[摘要]

The DNA of U937 cells exposed to two different apoptotic stimuli, namely the cocktail H₂O₂/3-aminobenzamide (3AB) and etoposide, was analyzed using field inversion gel electrophoresis (FIGE) as well as programmable, autonomously controlled electrode electrophoresis (PACE). The results obtained indicate that FIGE is not appropriate for sizing apoptotic DNA fragments. PACE appears to be more accurate and reliable and the results obtained with this technique strongly suggest that the 50 kb DNA fragments are directly excised from Mb-sized DNA fragments without the intermediate cleavage of 200–300 kb products.

[学科分类] 生物化学/生物物理

[关键词] Apoptosis;Necrosis;High molecular weight DNA fragmentation;Pulsed field gel electrophoresis;FIGE;field inversion gel electrophoresis;PACE;programmable;autonomously controlled electrode electrophoresis;HMW;high molecular weight;3AB;3-aminobenzamide;PFGE;pulsed field gel electrophoresis