Bafilomycin A1 induces apoptosis in PC12 cells independently of intracellular pH

[摘要]

PC12 cells growth-arrested with bafilomycin A₁ died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A₁-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A₁ than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH₄Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A₁, suggesting that bafilomycin A₁-induced apoptosis occurs independently of intracellular pH in PC12 cells.

[学科分类] 生物化学/生物物理

[关键词] Bafilomycin A1;Apoptosis;Vacuolar H+-ATPase;NH4Cl;PC12 cell;ATA;aurintricarboxylic acid;BCECF-AM;2′;7′-bis(carboxyethyl)-5(6)-carboxyfluorescein tetraacetoxymethylester;CHX;cycloheximide;DMSO;dimethylsulfoxide;FDA;fluorescein diacetate;HEPES;N-[2-hydroethyl]piperazine-N′-[2-ethanesulfonic acid];NOG;neurite outgrowth;NGF;nerve growth factor;PBS;phosphate-buffered saline