Modification of the targeting presequence of the bovine cytochrome P‐450scc precursor lifting tissue‐specific restrictions on its mitochondrial import

[摘要]

It has been found that a recombinant cytochrome P-450scc precursor supplemented with an extra MRGSH₆GIR sequence at the NH₂-terminus (6His-pP450scc) is imported into isolated rat liver and heart mitochondria as well as into yeast mitochondria. The import is coupled with proteolytic processing of the precursor resulting in the mature size form of cytochrome P-450scc. Modification of the targeting presequence responsible for its increased positive charge is supposed to lift the previously suggested tissue-specific restrictions on the pP450scc import into mitochondria.

[学科分类] 生物化学/生物物理

[关键词] Cytochrome P-450scc;Mitochondrial import;Addressing presequence;pP450scc;precursor of cytochrome P-450scc;6His-pP460scc;modified form of the cytochrome P-450scc precursor expressed in Escherichia coli;Ni-NTA agarose;agarose linked with Ni2+ nitrilo-tri-acetate