Complete synthesis of 3′‐sialyl‐N‐acetyllactosamine by regioselective transglycosylation

[摘要]

Transglycolytic synthesis of 3′-sialyl-N-acetyllactosamine by sequential use of β-galactosidase from Bacillus circulans and trans-sialidase from Trypanosoma cruzi was described. These reactions depicted the first complete synthesis of a biologically important oligosaccharide with high regioselectivity avoiding use of glycosyltransferases and NDP sugars.

[学科分类] 生物化学/生物物理

[关键词] Transglycosylation;Trans-sialidase;3′-Sialyl-N-acetyllactosamine;Sialyl-Lewisx;NDP sugars;nucleoside diphosphate sugars;3′-SLN;3′-sialyl-N-acetyllactosamine;D-GlcpNAc;2-acetamido-2-deoxy-d-glucopyranose;D-Glcp;d-glucopyranose;BcG;B. circulans β-galactosidase;TcTs;T. cruzi trans-sialidase;[14C]lactose;[d-(glucose)-1-14C]lactose;PBS;phosphate buffer saline;MU-NeuAc;MU;4-methylumbelliferone