Evidence that 3′‐phosphorylated polyphosphoinositides are generated at the nuclear surface: use of immunostaining technique with monoclonal antibodies specific for PI 3,4‐P2

[摘要]

Phosphatidylinositol (PI) 3,4-P₂ is a phosphoinositide that has been shown to be important for signal transduction in growth factor stimulation. We have produced monoclonal antibodies specific for PI 3,4-P₂, which were able to detect PI 3,4-P₂ generated in 293T cells treated with H₂O₂, or in MKN45/BD110 cells expressing activated PI 3-kinase in immunostaining. Prolonged treatment with 0.05% Tween 20 resulted in detection of staining not only at the plasma membrane, but also at the nuclear surface, indicating that 3′-phosphorylated phosphoinositides can be generated and function in the nucleus.

[学科分类] 生物化学/生物物理

[关键词] Phosphatidylinositol 3;4-P2;Monoclonal antibody;Immunostaining;Phosphatidylinositol 3-kinase