Identification of organic anion transporting polypeptide 4 (Oatp4) as a major full‐length isoform of the liver‐specific transporter‐1 (rlst‐1) in rat liver

[摘要]

A novel organic anion transporting polypeptide (Oatp)4¹ was isolated from rat liver that is 35 amino acids longer than the reported rat liver specific organic anion transporter (rlst)-1 and exhibits a 64% amino acid sequence identity with the human OATP-C (LST-1/OATP2; gene symbol SLC21A6). When expressed in Xenopus laevis oocytes, Oatp4 (Slc21a10) mediated polyspecific uptake of a variety of organic anions including taurocholate (K _m∼27 μM), bromosulfophthalein (K _m∼1.1 μM) and steroid conjugates. Based on nuclease protection analysis Oatp4 appears to be the predominant transcript in rat liver indicating that rlst-1 plays a minor role in overall hepatic organic anion uptake.

[学科分类] 生物化学/生物物理

[关键词] Liver transport;Bile salt;Sulfobromophthalein;Steroid conjugate;Thyroid hormone;Leukotriene C4;BSP;sulfobromophthalein;LST-1(OATP-C;OATP2);human liver specific transporter-1;Oatp;rat organic anion transporting polypeptide;OATP;human organic anion transporting polypeptide;OAT-K;rat kidney organic anion transporting polypeptide;PGT;prostaglandin transporter;rlst-1;rat liver specific transporter-1