ADAMTS‐1 cleaves a cartilage proteoglycan, aggrecan

[摘要]

A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu¹⁸⁷¹–Leu¹⁸⁷² bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo.

[学科分类] 生物化学/生物物理

[关键词] Aggrecan;Disintegrin and metalloproteinase thrombospondin;Extracellular matrix;Cartilage degradation;ADAM;disintegrin and metalloproteinase;CS;chondroitin sulfate;DMEM;Dulbecco's modified Eagle's medium;ECM;extracellular matrix;IGD;interglobular domain;KS;keratan sulfate;MMP;matrix metalloproteinase;PAGE;polyacrylamide gel electrophoresis;SDS;sodium dodecyl sulfate;TSP;thrombospondin