Efficient phage display of polypeptides fused to the carboxy‐terminus of the M13 gene‐3 minor coat protein

[摘要]

We report that, contrary to common belief, polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein are functionally displayed on the phage surface. In a phagemid display system, carboxy-terminal fusion through optimized linker sequences resulted in display levels comparable to those achieved with conventional amino-terminal fusions. These findings are of considerable importance to phage display technology because they enable investigations not suited to amino-terminal display, including the study of protein–protein interactions requiring free carboxy-termini, functional cDNA cloning efforts, and the display of intracellular proteins.

[学科分类] 生物化学/生物物理

[关键词] M13 bacteriophage;Carboxy-terminus;Phage display;Minor coat protein;P3;protein-3;the gene-3 minor coat protein of M13 phage;P8;protein-8;the gene-8 major coat protein of M13 phage;truncated P3;P3 residues 257–406