A novel fluorescent probe diphenyl‐1‐pyrenylphosphine to follow lipid peroxidation in cell membranes

[摘要]

Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP=O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.

[学科分类] 生物化学/生物物理

[关键词] Lipid peroxidation;Polymorphonuclear leukocyte;Imaging;Diphenyl-1-pyrenylphosphine;Fluorescent probe;tBuOOH;tert-butyl hydroperoxide;CCD;charge-coupled device;DCFH-DA;dichlorodihydrofluorescein diacetate;DHR;dihydrorhodamine;DMSO;dimethyl sulfoxide;DOPC;dioleoyl phosphatidylcholine;DPPP;diphenyl-1-pyrenylphosphine;DPPP=O;diphenyl-1-pyrenylphosphine oxide;FBS;fetal bovine serum;HBSS;Hanks’ balanced salt solution;MeLOOH;methyl linoleate hydroperoxide;PBS;phosphate-buffered saline;PMA;phorbol 12-myristate 13-acetate;PMN;polymorphonuclear leukocyte