Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target

[摘要]

A sensitive cellular assay system for RNA interference was developed using the firefly luciferase gene as target. RNA interference was noted not only in Drosophila cultured cells but Chinese hamster cells (CHO-K1) as well, although double-stranded RNA required for the latter was 2500 times more than for the former. Cognate double-stranded RNA as short as 38 bp was found to be still capable of inducing RNA interference in Drosophila cultured cells.

[学科分类] 生物化学/生物物理

[关键词] RNA interference;Double-stranded RNA;Chinese hamster;Cultured cell;Drosophila;RNAi;RNA interference;dsRNA;double-stranded RNA;luc;luciferase;Dfz2;Drosophila melanogaster frizzled 2;PKR;dsRNA-responsive protein kinase