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Use of an affinity proteomics approach for the identification of low‐abundant bacterial adhesins as applied on the Lewisb‐binding adhesin of Helicobacter pylori
[摘要]

Microbial attachment to host cell surfaces is considered to be the first essential step for colonization and infection. In most known cases, attachment is mediated by a specific protein–carbohydrate interaction. We have used a carbohydrate-containing crosslinking probe to select bacterial surface adhesins for trypsin digestion, MALDI-TOF mass spectrometry and identification against genome sequence. The present paper describes this functional proteomics approach for identification of the recently cloned low-abundant Lewisb-binding adhesin of Helicobacter pylori. Protein identification was obtained through the enrichment of approximately 300 fmol of adhesin from solubilized cells.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Bacterial adhesin;Low-abundance protein;Proteomics;Protein–carbohydrate interaction;Helicobacter pylori [时效性] 
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