Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis

[摘要]

We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP-1. XCIRP-1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP-1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP-1 specific as they could be rescued by sense transcript. Suppression of XCIRP-1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP-1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim-1 and WT1 genes. These results suggest that XCIRP-1 is required for the specification and morphogenetic lineage migration of the pronephros.

[学科分类] 生物化学/生物物理

[关键词] Cold inducible RNA binding protein;cDNA cloning;Pronephros;Morphogenetic movement;Xenopus laevis;CIRP;cold inducible RNA binding protein;RA;retinoic acid;Xlim-1;LIM class homeobox containing gene;WT1;Wilm's tumor suppressor gene;Xbra;Xenopus brachyury;EF-1α;elongation factor-1 α;AC;animal cap