Binding to 14‐3‐3 proteins is not sufficient to inhibit nitrate reductase in spinach leaves

[摘要]

To assess the role of 14-3-3 proteins in the magnesium-dependent inhibition of nitrate reductase (NR) we tested the effect of magnesium on NR binding to 14-3-3s by coimmunoprecipitation and gel filtration. The stability of the 14-3-3 complex of NR was, unlike its activity, unaffected by magnesium. We therefore conclude that binding to 14-3-3s per se does not inhibit NR. Magnesium inhibited 14-3-3-bound NR much more strongly than 14-3-3-free NR. 14-3-3s possibly reinforce NR inhibition by magnesium.

[学科分类] 生物化学/生物物理

[关键词] 14-3-3 Protein;Nitrate reductase;Protein phosphorylation;Protein–protein interaction;Enzyme inhibition