Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides 1

[摘要]

Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.

[学科分类] 生物化学/生物物理

[关键词] Endotoxin;Structure;Mass spectrometry;Serology;Bordetella hinzii;CE;capillary electrophoresis;ELISA;enzyme-linked immunosorbent assay;ES/MS;electrospray mass spectrometry;FAB;fast atom bombardment;Fuc2NAc4NMe;2-acetamido-4-N-methyl-2;4;6-deoxy-galactose;GalNA;galactosaminuronic acid;GlcA;glucuronic acid;GlcNAc;N-acetyl glucosamine;GC;gas chromatography;Hep;heptose;LPS;lipopolysaccharide;mAb;monoclonal antibody;MALDI;matrix-assisted laser desorption/ionization;Man2NAc3NAcA;2-acetamido-3-acetamido-2;3-dideoxy-mannuronic acid;NMR;nuclear magnetic resonance;PDMS;plasma desorption mass spectrometry;PEA;phosphoryl ethanolamine;PPEA;pyrophosphoryl ethanolamine