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Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O‐sulfation of preprophytosulfokine variants in vitro
[摘要]

An in vitro assay system to detect tyrosylprotein sulfotransferase (TPST) activity of higher plant cells was established, using synthetic oligopeptides based on the deduced amino acid sequence of a phytosulfokine-α (PSK-α) precursor. TPST activity was found in microsomal membrane fractions of rice, asparagus and carrot cells and it was confirmed that acidic amino acid residues adjacent to the tyrosine residues of acceptor peptides were essential to the sulfation reaction. The asparagus TPST exhibited a broad pH optimum of 7.0–8.5, required manganese ions for maximal activity and appeared to be a membrane-bound protein localized in the Golgi apparatus. These enzymes should be defined as a new class of plant sulfotransferases that catalyze tyrosine O-sulfation of a PSK-α precursor and other unknown proteins.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Phytosulfokine-α;Tyrosine O-sulfation;Tyrosylprotein sulfotransferase;PAPS;3′-phosphoadenosine 5′-phosphosulfate;PP-PSK;preprophytosulfokine;PSK-α;phytosulfokine-α;TPST;tyrosylprotein sulfotransferase [时效性] 
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