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Colecystokinin octapeptide CCK‐8 and carbachol reduce [32P]orthophosphate labeling of phosphatidylcholine without modifying phospholipase D activity in rat pancreatic acini
[摘要]

We have studied phospholipase D activation in [32P]orthophosphoric acid-prelabeled rat pancreatic acini by measuring the formation of 32P-phosphatidylalcohols as stimulated in the presence of ethanol or butanol. A small but significant and time-dependent basal accumulation of [32P]phosphatidylethanol and [32P]phosphatidylbutanol was detected, which was further stimulated by phorbol myristate acetate, orthovanadate and pervanadate. However, the secretagogues cholecystokinin octapeptide and carbachol did not enhance basal accumulation of 32P-phosphatidylalcohol, yet they decreased [32P]phosphatidylcholine content and stimulated the generation of [32P]phosphatidic acid. Our results stress the need to examine the transphosphatidylation reaction as well as agonist effects on the synthesis of phosphatidylcholine in order to assess unambiguously phospholipase D activity.

[发布日期]  [发布机构] 
[效力级别]  [学科分类] 生物化学/生物物理
[关键词] Phospholipase D;Phosphatidylcholine;Phosphatidylethanol;Pancreatic acinus;CCK-8;cholecystokinin octapeptide (26–33);Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2;Cch;carbachol;PMA;phorbol myristate acetate [时效性] 
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