Inositol 1,4,5‐trisphosphate receptor associated with focal contact cytoskeletal proteins

[摘要]

The linkage between inositol 1,4,5-trisphosphate (IP₃) receptors (IP₃Rs) and cytoskeletal proteins is considered to be important in cell function. In the present study, the association of IP₃R subtypes with cytoskeletal proteins was examined using monoclonal antibodies specific to each IP₃R subtype. We found that IP₃R type 2 colocalized with talin, a focal contact cytoskeletal protein. IP₃R type 2 exhibited a patchy distribution in the peripheral cytoplasm differently from type 1 and type 3 IP₃R. Furthermore, IP₃R subtypes co-immunoprecipitated with talin, vinculin and α-actin, but not α-actinin or paxillin.

[学科分类] 生物化学/生物物理

[关键词] Inositol 1;4;5-trisphosphate receptor;Cytoskeletal protein;Talin;Focal contact;Immunocytochemistry;Smooth muscle cell;IP3;inositol 1;4;5-trisphosphate;IP3R;IP3 receptor;ER;endoplasmic reticulum;PLC;phospholipase C;PIP2;phosphatidylinositol bisphosphate;FITC;fluorescein isothiocyanate;SDS-PAGE;sodium dodecyl sulfate-polyacrylamide gel electrophoresis