Alternative 5′‐exons of the mouse cAMP‐dependent protein kinase subunit RIα gene are conserved and expressed in both a ubiquitous and tissue‐restricted fashion

[摘要]

The activity of cAMP-dependent protein kinase is controlled by its regulatory subunits. Mouse RIα regulatory subunit expression is initiated from five different non-coding 5′-regions (exons 1a, 1b, 1c, 1d and 1e). This organization appears to be conserved among species. All mouse tissues accumulate exon 1a and 1b transcripts and most contain more 1b than 1a, except brain, heart and oesophagus. Exon 1d and 1e transcripts are found in several tissues, while exon 1c is testis-specific. All five transcripts are in RIα-rich tissues: gonads and adrenal glands.

[学科分类] 生物化学/生物物理

[关键词] cAMP-dependent protein kinase;5′-Untranslated region;Alternative transcript;PKA;cAMP-dependent protein kinase;5′-RACE;5′-rapid amplification of cDNA ends;RT-PCR;reverse transcription-polymerase chain reaction;GAPDH;glyceraldehyde-3-phosphate dehydrogenase;UTR;untranslated region